Pavlo Hrab

2023 - Present

Built a Nextflow pipeline to clean and dereplicate 19,720 Acidobacteriota MAGs from public data, and mined the result for biosynthetic gene clusters across every biome the phylum occupies.

focus

Acidobacteriota · 19,720 MAGs

source

public metagenomes, all biomes

stack

Nextflow · CheckM2 · GUNC · dRep · GTDB-Tk · antiSMASH · BiG-SCAPE

output

BGC catalogue, 2 released tools

2022 - Present

Planned and led a year-long isolation study of sponge-associated bacteria. Used metagenomics and metatranscriptomics to predict what the uncultured lineages needed, designed the media from those predictions, and ran the cultivation myself.

focus

Geodia barretti microbiome

source

deep-sea sponge, metagenomes + metatranscriptomes

stack

functional annotation · media design · bench cultivation

output

isolates, new physiological properties

2024 - Present

Analysed 488 marine Acidobacteriota genomes across 16 families to separate genuine host adaptation from shared ancestry, using Pfam-based feature matrices, phylogenomics and machine learning.

focus

marine Acidobacteriota · 488 genomes, 16 families

source

host-associated and free-living marine genomes

stack

pyhmmer · Pfam-A · phylogenomics · random forest

output

domain matrix, enrichment results, pfam_tools